Characteristics of quinolone and beta-lactam antibiotic resistance in Salmonella isolated from raw meat in Ho Chi Minh City

In this study, a total of 380 raw meat samples (pork, beef, and chicken) were collected randomly from traditional markets in Ho Chi Minh City (Vietnam). Salmonella strains were isolated by cultivation methods according to ISO 6579-1:2017, subsequently confirmed by PCR method (TCVN 8342:2010). These used test antibiotic susceptibility in six kinds antibiotics belonging two groups quinolones (nalidixic, ciprofloxacin, and ofloxacin), β-lactam (ampicillin, amoxicillin, cephalexin) together with the detection their resistant genotypes was estimated Kirby-Bauer multiplex PCR. It noted that the proportion spp. contaminated 42.37% (161/380). Specifically, found 50.00 % (63/126), 49.62% (65/131), and 26.83% (33/123) pork, chicken, beef samples, respectively. noticed all isolated types antibiotics. The highest 22.98% for ampicillin (AM), followed 10.56% for nalidixic acid (NA). proportions amoxicillin/acid clavulanic (AMC), ceftazidime (CAZ), ciprofloxacin (CIP), ofloxacin (OPX) resistant strains remarkably low (< 10%). There 37/161 (22.98%) carrying TEM genes 5/161 (3.11%) carrying CTX genes. On other hand, there no strain carrying SHV Four quinolone-resistant including gyrA, gyrB involved nalidixic acid resistance, parC, parE resistance detected in all also carried β-lactamase encoding is important note that blaTEM-gyrA/B-parC/E simultaneously tested strains. While the proportion containing blaCTX-gyrA/B-parC/E 40.54%, proportion of blaTEM/CTX-gyrA/B-parC/E 5.41%. results revealed that raw might be are highly β-lactam antibiotics quinolones. Henceforth, it necessary establish monitoring surveillance programs on contamination sensitivity Vietnam protect consumers’ health. study provided direct evidence and antibiotic situation City.